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《医学前沿(英文)》 2017年 第11卷 第3期 页码 410-422 doi: 10.1007/s11684-017-0527-6
Aberrant expression of annexin A2-S100A10 heterotetramer (AIIt) associated with PML/RARα fusion protein causes lethal hyperfibrinolysis in acute promyelocytic leukemia (APL), but the mechanism is unclear. To facilitate the investigation of regulatory association between ANXA2 and promyelocytic leukemia/retinoic acid receptor a (PML/RARα) fusion protein, this work was performed to determine the transcription start site of ANXA2 promoter with rapid amplification of 5′-cDNA ends analysis. Zinc-induced U937/PR9 cells expressed PML/RARα fusion protein, and resultant increases in ANXA2 transcripts and translational expressions of both ANXA2 and S100A10, while S100A10 transcripts remained constitutive. The transactivation of ANXA2 promoter by PML/RARα fusion protein was 3.29±0.13 fold higher than that by control pSG5 vector or wild-type RARα. The overexpression of ANXA2 in U937 transfected with full-length ANXA2 cDNA was associated with increased S100A10 subunit, although S100A10 transcripts remained constitutive. The tPA-dependent initial rate of plasmin generation (IRPG) in zinc-treated U937/PR9 increased by 2.13-fold, and cell invasiveness increased by 27.6%. Antibodies against ANXA2, S100A10, or combination of both all remarkably inhibited the IRPG and invasiveness in U937/PR9 and NB4. Treatment of zinc-induced U937/PR9 or circulating APL blasts with all-trans retinoic acid (ATRA) significantly reduced cell surface ANXA2 and S100A10 and associated reductions in IRPG and invasiveness. Thus, PML/RARα fusion protein transactivated the ANXA2 promoter to upregulate ANXA2 and accumulate S100A10. Increased AIIt promoted IRPG and invasiveness, both of which were partly abolished by antibodies against ANXA2 and S100A10 or by ATRA.
关键词: annexin A2-S100A10 heterotetramer PML/RARα fusion protein plasmin cell invasion acute promyelocytic leukemia
Regulatory mechanism and functional analysis of
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《医学前沿(英文)》 2017年 第11卷 第1期 页码 87-96 doi: 10.1007/s11684-016-0469-4
S100A9, a calcium-binding protein, participates in the inflammatory process and development of various tumors, thus attracting much attention in the field of cancer biology. This study aimed to investigate the regulatory mechanism of and its function involvement in APL. We used real-time quantitative PCR to determine whether PML/RARα affects the expression of in NB4 and PR9 cells upon ATRA treatment. ChIP-based PCR and dual-luciferase reporter assay system were used to detect how PML/RARα and PU.1 regulate promoter activity. CCK-8 assay and flow cytometry were employed to observe the viability and apoptosis of NB4 cells when was overexpressed. Results showed that was an ATRA-responsive gene, and PML/RARα was necessary for the ATRA-induced expression of in APL cells. In addition, PU.1 could bind to the promoter of , especially when treated with ATRA in NB4 cells, and promote its activity. More importantly, overexpression of induced the apoptosis of NB4 cells and inhibited cell growth. Collectively, our data indicated that PML/RARα and PU.1 were necessary for the ATRA-induced expression of in APL cells. Furthermore, promoted apoptosis in APL cells and affected cell growth.
关键词: S100A9 S100A9 PU.1 PML/RARα ATRA APL
孙学用
《中国工程科学》 2007年 第9卷 第12期 页码 94-96
Preface of special issue of the 10 th International Conference on CO 2 Utilization
Changjun LIU,
《化学科学与工程前沿(英文)》 2010年 第4卷 第2期 页码 109-109 doi: 10.1007/s11705-009-0258-6
《医学前沿(英文)》 2022年 第16卷 第6期 页码 969-974 doi: 10.1007/s11684-021-0912-z
关键词: severe malaria nigeria malaria mortality PfPR2-10 intermediate malaria transmission
赵金省,高建英,肖曾利
《中国工程科学》 2013年 第15卷 第10期 页码 66-70
《环境科学与工程前沿(英文)》 2022年 第16卷 第9期 doi: 10.1007/s11783-022-1552-5
• The airborne bacteria of Mexico City are representative of urban environments.
关键词: Airborne bacteria Urbanisation PM10 Mexico City Microbiome
Fabrication of MIL-100(Fe)@SiO
Yinlong Hu,Shuang Zheng,Fumin Zhang
《化学科学与工程前沿(英文)》 2016年 第10卷 第4期 页码 534-541 doi: 10.1007/s11705-016-1596-9
关键词: metal-organic frameworks heterogeneous catalysis magnetically recoverable catalysts core-shell structure acetalization
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《医学前沿(英文)》 2016年 第10卷 第4期 页码 444-450 doi: 10.1007/s11684-016-0471-x
The incidence rate of pancreatic neuroendocrine neoplasms (pNENs) has increased rapidly in recent years. However, the clinicopathological characteristics of pNENs are poorly understood. Medical records of patients who underwent surgery and were confirmed as pNENs by pathological examination from January 2003 to February 2015 in Qilu Hospital were reviewed retrospectively. A total of 100 patients, 36 males and 64 females, were included with a mean operation age of 46.26±13.41 years. Among the 100 cases, 76 had insulinomas and 24 had non-functional pNENs. Tumor size ranged from 0.5 cm to 9 cm, and the mean size was 2.20±1.40 cm. The percentages of TNM stages I, II, III, and IV tumors were 89.0%, 8.0%, 0.0%, and 3.0%, respectively. Based on the WHO classification, pNENs were classified into three grades: G1, G2, and G3. G1, G2, and G3 tumors were confirmed in 72.9%, 23.7%, and 3.4% patients, respectively. The positive rates of CgA and Syn immunohistochemical staining were 94.5% (69/73) and 100% (74/74), respectively. Compared with insulinoma, non-functional pNENs have larger tumor sizes, more advanced TNM staging, a higher Ki-67 index, and a higher rate of liver metastasis (P<0.05). In conclusion, pNENs are heterogeneous tumors with varying clinical manifestations, diverse tumor biological characteristics, and different prognoses. Non-functional pNENs present a more aggressive behavioral model and have poorer prognosis than functional pNENs.
关键词: pancreatic neuroendocrine neoplasms clinicopathological characteristic Ki-67 liver metastasis outcomes
NO hydrogenation to NH over FeCu/TiO catalyst with improved activity
《化学科学与工程前沿(英文)》 2023年 第17卷 第12期 页码 1973-1985 doi: 10.1007/s11705-023-2364-2
关键词: NO hydrogenation synthetic ammonia 10Fe-xCu/TiO2 high selectivity
《化学科学与工程前沿(英文)》 2023年 第17卷 第7期 页码 930-941 doi: 10.1007/s11705-023-2309-9
关键词: lignin electrospinning heterojunctions photo-Fenton catalysis wastewater remediation
PM10 emissions from industrial coal-fired chain-grate boilers
Xinghua Li, Junzan Han, Lei Duan
《环境科学与工程前沿(英文)》 2017年 第11卷 第6期 doi: 10.1007/s11783-017-0966-y
关键词: coal-fired chain-grate boiler PM10 size distribution particulate emission control devices size-dependent collection efficiency
Real drive cycles analysis by ordered power methodology applied to fuel consumption, CO
Antonia de los Santos López, Jesús Álvarez-Flórez
《环境科学与工程前沿(英文)》 2021年 第15卷 第1期 doi: 10.1007/s11783-020-1296-z
关键词: Cycle Analysis by Ordered Power (CAbOP) Micro and macro models Real drive cycle NOx/PM10/CO2 emissions Wheel mechanical power domain Worldwide Harmonized Light-Duty Vehicles Test Cycle (WLTC)
Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice
《医学前沿(英文)》 页码 957-971 doi: 10.1007/s11684-023-0988-8
关键词: DNAH10 mice motile cilia mutation primary ciliary dyskinesia
《医学前沿(英文)》 2022年 第16卷 第4期 页码 596-609 doi: 10.1007/s11684-021-0868-z
关键词: innate immune checkpoint Siglec10 kidney renal clear cell carcinoma
标题 作者 时间 类型 操作
Annexin A2-S100A10 heterotetramer is upregulated by PML/RARα fusion protein and promotes plasminogen-dependent
null
期刊论文
Preface of special issue of the 10 th International Conference on CO 2 Utilization
Changjun LIU,
期刊论文
Cross-sectional prevalence and pattern of non-anaemia severe malaria among 2–10 year olds in Sokoto in
期刊论文
associated with particulate matter from a highly urbanised metropolis: A potential risk to the population’s
期刊论文
Analysis of 100 consecutive cases of resectable pancreatic neuroendocrine neoplasms: clinicopathological
null
期刊论文
Lignin-based electrospun nanofiber membrane decorated with photo-Fenton Ag@MIF-100(Fe) heterojunctions
期刊论文
Real drive cycles analysis by ordered power methodology applied to fuel consumption, CO
Antonia de los Santos López, Jesús Álvarez-Flórez
期刊论文